Wednesday, July 24, 2019
Leadership development paper Coursework Example | Topics and Well Written Essays - 250 words
Leadership development paper - Coursework Example I am also success oriented, flexible, adaptable, trustworthy, and loyal. These strengths explain my level of success in leadership and managerial capacities. The self-assessment reports also identify low self-esteem and vulnerability to peer influence. While low self-esteem is likely to reduce my level of motivation, especially in challenging roles, vulnerability to peer influence is a threat to my leadership toward realization of set objectives. My other challenges are low ability to manage pressure and weak conflict resolution. In conflict resolution, I majorly apply accommodation and avoidance but these limit my ability to win in conflict resolutions. My leadership development goals include improving my level of self-esteem, improving my level of independence, and my conflict resolution skills. I plan to attend leadership seminars and to be mentored within the next six months. I will then conduct another self-assessment test to evaluate by score in the three areas of
Tuesday, July 23, 2019
Goals in life Essay Example | Topics and Well Written Essays - 750 words
Goals in life - Essay Example Starting with what you know involves getting to know yourself a little better without all the hang-ups and expectations of the world you were brought up in. To get to know yourself better, you have to sit down and really think about whatââ¬â¢s important to you, what your morals are and what you feel is ethically correct. This is all about those intangible things that are meaningful to you. If youââ¬â¢re thinking about how impressive a 15 bedroom house would be to your friends and family, youââ¬â¢re on the wrong track but if youââ¬â¢re thinking about how it might be necessary to have 15 bedrooms in order to house all those children you want to have, you might be onto something. Follow the thought a little deeper and you might discover that what is important to you is that you have a close relationship with a number of different people. The next step after figuring out whatââ¬â¢s really important to you is to experiment with those areas that you arenââ¬â¢t all that familiar with. Do you like to do things outdoors? If youââ¬â¢ve grown up in the inner city, this might be a very difficult question to answer. While youââ¬â¢re young is the time to experiment with different ways of living, different ways of looking at the world and different ideas of what you might wish to do in life. Talk with people who are from different countries, different backgrounds and different socio-economic classes. You might learn something about yourself and you will certainly gain a greater appreciation for the diversity of the world around you. You may discover that your inner city dreams of the 15 bedroom house meant that you want close relationships with people but that you also desire more personal space, meaning an office job will not make you happy. Finally, knowing what is really important to you and having had the chance to experiment with a variety of viewpoints, you are ready to begin setting your goals. Envision what your life might look like in 20 years
Monday, July 22, 2019
J.C. Penneyââ¬â¢s ââ¬ÅFair and Squareââ¬Â Pricing Strategy Essay Example for Free
J.C. Penneyââ¬â¢s ââ¬Å"Fair and Squareâ⬠Pricing Strategy Essay Retailing is hard, and thatââ¬â¢s what Steve [Jobs] said to me when we started stores at Apple. ââ¬â Ron Johnson, CEO, J.C. Penney1 It was August 2012 and the release of second quarter earnings was looming for Ron Johnson, the chief executive officer of J.C. Penney, one of Americaââ¬â¢s first department stores. Johnson, HBS ââ¬â¢84, had intimated to Wall Street that the retailerââ¬â¢s second quarter results were likely to miss expectations again, following dismal first quarter results that had sent the companyââ¬â¢s stock price careening to less than half of its February 2012 value of a share. The Q1 news released in May was grim: a $163 million loss, same store revenue down 19%, and the number of customers shopping in J.C. Penney stores down 10%. These results were particularly disheartening given the companyââ¬â¢s radical repositioning of its business model and its brand in February 2012. The centerpiece of the repositioning initiative was a switch from J.C. Penneyââ¬â¢s existing high-low pricing strategy, in which the retailer ran frequent sales to offer customers deep discounts off of its higher list prices, to a new strategy the company dubbed ââ¬Å"Fair and Squareâ⬠pricing. ââ¬Å"Fair and Squareâ⬠pricing was meant to simplify J.C. Penneyââ¬â¢s pricing structure and make it more straightforward for customers to shop. It offered great prices every day, with less frequent price promotions. The company touted its new pricing strategy as offering ââ¬Å"no games, no gimmicksâ⬠and invited consumers to ââ¬Å"do the mathâ⬠to see how it offered them cheaper prices on a regular basis with less hassle. Moving away from high-low pricing was a massive shift for J.C. Penney. In 2011, the retailer spent $1.2 billion to execute 590 different sales events and promotions2 and generated 72% of its $17.3 billion in annual revenue from products sold at steep discounts of more than 50% off of the initial list price.3 Wall Street was initiallyà supportive of the companyââ¬â¢s plans for change. Investors, who sent J.C. Penneyââ¬â¢s stock soaring up 24% following the announcement of the new pricing plan, viewed it as a way for J.C. Penney to escape the ruthless downward spiral of escalating price promotions that gripped Americaââ¬â¢s retailers struggling to survive the economic recession. But by mid-summer 2012, customers and shareholders appeared to be voting with their feet, leaving the retailer in droves. Was Johnsonââ¬â¢s new pricing strategy misguided or was it just a matter of time before customers fully embraced it? Johnson was under enormous pressure to turn things around quickly as the all-important back-to-school and holiday shopping seasons were imminent. Many voices were calling on him to consider changing the pricing strategy again. ________________________________________________________________________________________________________________ HBS Professor Elie Ofek and Professor Jill Avery (Simmons School of Management) prepared this case. This case was developed from published sources. HBS cases are developed solely as the basis for class discussion. Cases are not intended to serve as endorsements, sources of primary data, or illustrations of effective or ineffective management. Copyright à © 2012, 2013 President and Fellows of Harvard College. To order copies or request permission to reproduce materials, call 1-800-5457685, write Harvard Business School Publishing, Boston, MA 02163, or go to www.hbsp.harvard.edu/educators. This publication may not be digitized, photocopied, or otherwise reproduced, posted, or transmitted, without the permission of Harvard Business School. This document is authorized for use only by Jack Cherewatti in MKTG MGMT taught by S. Adam Brasel Boston College from November 2014 to May 2015. For the exclusive use of J. Cherewatti 513-036 J.C. Penneyââ¬â¢s ââ¬Å"Fair and Squareâ⬠Pricing Strategy Company Background Johnson was at the helm of what at one time was considered Americaââ¬â¢s mostà venerated department store. Once the largest department store chain in the country with over 2,000 stores, as of 2012, the 110 year old retailer operated 1,100 stores, claiming to serve more than half of Americaââ¬â¢s households with 41 million square feet of retail space. Founded by James Cash Penney in 1902, the companyââ¬â¢s first outlet was opened in a Wyoming mining town under the name ââ¬Å"The Golden Rule,â⬠that signified its philosophy of treating customers the way Penney himself wished to be treated. Johnson believed that his ââ¬Å"Fair and Squareâ⬠pricing plan corresponded to the founderââ¬â¢s beliefs, ââ¬Å"Now if you go back to the founding of this company, James Cash Penney believed in everyday fair prices. He said, ââ¬ËWe donââ¬â¢t mark goods up just to mark them down. We donââ¬â¢t believe in sales.ââ¬â¢Ã¢â¬ 4 The company enjoyed years of rapid growt h and expansion. By its 50th anniversary, annual sales exceeded $1 billion. It initially offered consumers one stop shopping as a mass merchandiser, selling soft goods, such as clothing, as well as hard goods, such as appliances, hardware, electronics, and sporting goods. Its retail business was joined by a mail order catalog in 1963 and an ecommerce website in 1998. However, following tough times in the 1980ââ¬â¢s, the company reorganized, phasing out its hard goods lines and refocusing on its soft goods to become a fashion oriented department store. But by its 100th anniversary, the company appeared to be running out of steam. Price-oriented mass merchandisers, such as Walmart and Target, had garnered the lower end of the market, while higher end department stores, such as Macyââ¬â¢s and Nordstromââ¬â¢s, were catering to the upwardly mobile middle class. Although the economic recession of 2008 was difficult for all retailers due to consumersââ¬â¢ increasing frugality, middle market retailers, like J.C. Penney and Sears, were hit the hardest. By 2011, J.C. Penneyââ¬â¢s stores were old, often disorganized, and faded, and the brand and its merchandise were starting to feel dated. About 400 of its stores were located in small towns, such as Alpena, Michigan with a population of a little over 10,000. In such towns, there were often only few, if any, other department stores. The remaining 700 or so stores were located in major metropolitan areas, often in suburban malls, such as the Northshore Mall in Peabody, Massachusetts (15 miles north of Boston). Following years of store closings, sales malaise, declining market share,à slumping earnings, and weak stock market performance, activist investor and hedge fund manager, William Ackman (HBS ââ¬â¢92) obtained an 18% majority shareholder position in the company in 2010ââ¬â2011. He was determined to turn J.C Penney around and extract its value, much of which was locked up in its vast real estate holdings that were estimated to be worth $11 billion.5 J.C. Penney owned 400 of its retail stores and paid low rents (an average of less than $5 per square foot) for the remainder. Specialty stores like Gap paid much higher rents (around $40 per square foot) for their retail space.6 Looking to shake up the company, Ackman was instrumental in luring Johnson to take the CEO position. Johnson was a big catch. In the 1990s, he was vice president of merchandising at Target where he helped transform the mass merchandiser into a hot retail brand selling stylish yet affordable products. During his time there, Johnson negotiated a contract with designer Michael Graves, beginning Targetââ¬â¢s profitable partnerships with high end designers, which enhanced its brand image as a chic, fashion-forward retailer. Starting in 2000, he worked with Steve Jobs to develop the wildly successful Apple retail stores. Johnson was the brainchild behind the ââ¬Å"Genius Barsâ⬠concept, a free technical help and support area staffed by knowledgeable customer service representatives, widely touted as one of the most innovative retail concepts of the last decade. Johnson was regarded by many as creative and determined; according to a friend, ââ¬Å"What people loved more about him than his talent was his persistence. He was just relentless.â⬠7 Johnsonââ¬â¢s deep retail experience combined with his wholesome charisma and boyish enthusiasm made him the perfect change agent. The media dubbed him the ââ¬Å"Steve Jobs of the retail industryâ⬠and on the day his appointment was announced J.C. Penneyââ¬â¢s stock jumped 18%. An Industry under Pressure J.C. Penneyââ¬â¢s 2011 sales were lower than they were in the 1990s and the retail landscape was getting more competitive. Department stores, in particular, were under increased pressure. New retail formats, such as big box retailers like Walmart that operated free standing supercenters selling mass merchandise and small specialty stores like Gap and J. Crew that were located in shopping malls and offered specialized merchandise, were squeezing department stores out of the market (see Exhibit 2). An emerging challenge came from large international clothing retailers, such as HM and ZARA, that were aggressively entering the U.S. market. These retailers relied on shorter product life cycles and partnerships with top designers to offer fast-fashion merchandise at relatively low prices. Johnson explained the challenge as he stepped into his new role: Over the past 30 years the department store has become a less relevant part of the retail infrastructure, largely because of decisions the stores have made. As America exploded with big box and specialty stores and new shopping formats, department stores abdicated their unique role instead of engaging the competition. They retreated from categories and assortments that made them distinctive. Department stores were once the most popular places for Americans to shop, offering distinctive merchandise in elegant settings that provided special services, such as tearooms, salons, and on-site tailoring, and served as social hubs. Johnson reminisced, ââ¬Å"In the golden age of department stores, Americaââ¬â¢s families came for more than just to shop. They were able to have fun experiences and were offered a range of useful services. . . . If we want to transform the department store, we have to understand what happened. These stores were a pillar of the community.â⬠9 Johnson, unlike others, believed that department stores could be revived. ââ¬Å"Thereââ¬â¢s no reason department stores canââ¬â¢t flourish. They can be peopleââ¬â¢s favorite place to shop. Theyââ¬â¢ve got all these strategic advantagesââ¬âthe lowest cost of real estate, exceptional access to merchandise, scale to create enormous marketing power, colocation with specialty stores. And people like storesà with huge assortments and one-stop shopping.â⬠10 J.C. Penneyââ¬â¢s performance had been lackluster for quite some time, and the retailer was losing market share even within the shrinking department store channel (see Exhibits 3 and 4). Competitors Macyââ¬â¢s and Kohlââ¬â¢s were nipping at J.C. Penneyââ¬â¢s business from both the high and low end. The average J.C. Penney customer only visited a store four times per year and sales per square foot ($156) were low compared to those of its competitors and the specialty stores Johnson hoped to emulate (Gap $30011, Apple $5,626 in sales per square foot). 12 Department stores and big box stores had increased their promotional budgets since the outbreak of the Great Recession in 2007 and most used blockbuster sales, coupons, and frequent price promotion to drive purchases. According to consulting firm A.T. Kearney, more than 40% of the items Americans bought in 2011 were bought on sale, up from 10% in 1990.13 Many retailers were eager to wean shoppers off of the big discounts that had become commonplace. Competition was also increasing from online retailing. Yet Johnson believed brick and mortar stores were still relevant, ââ¬Å"Physical stores are still the primary way people acquire merchandise and I think that will be true 50 years from now. . . . A store has got to be much more than a place to acquire merchandise. Itââ¬â¢s got to help people enrich their lives. If the store just fulfills a specific product need, itââ¬â¢s not creating new types of value for the consumer. Itââ¬â¢s transacting. Any website can do that.â⬠14 Many of J.C. Penneyââ¬â¢s largest competitors, such as Macyââ¬â¢s, seemed to have a differentà view and were investing heavily in their e-commerce operations and in catering to what they called the ââ¬Å"omnichannelâ⬠consumer, who accessed the retailer through the web, on mobile devices or in physical stores (often as part of the same purchase decision). Although it had been a pioneer in multi-channel commerce, with 2001 combined cata log and web sales of nearly $3.4 billion, J.C. Penneyââ¬â¢s ecommerce sales had stagnated over the last three years while those of Macyââ¬â¢s and Kohlââ¬â¢s had grown substantially during the same time frame.15 (See Exhibit 5 for E-commerce sales growth). J.C. Penneyââ¬â¢s Radical Makeover Following his appointment in November 2011, Johnson determined that nothing short of a complete overhaul would solve J.C. Penneyââ¬â¢s problems. Just two months after taking the helm, Johnson and his newly recruited leadership team, culled largely from Apple and Target, announced a radical repositioning of the J.C. Penney business model and brand. Following the announcement, Forbes magazine dubbed J.C. Penney the most interesting retail story of the year, proclaiming, ââ¬Å"This week, Johnson took a sledgehammer to the J.C. Penney way of doing business. Itââ¬â¢s the most exciting thing Iââ¬â¢ve seen in retail since Apple opened stores, again with Johnson at the helm.â⬠16 The turnaround plan evoked J.C. Penneyââ¬â¢s founding spirit, and Johnson declared it a reclamation of the companyââ¬â¢s heritage. J.C. Penneyââ¬â¢s website announced, ââ¬Å"Over 100 years ago, James Cash Penney founded his company on the principle of treating customers the way he wanted to b e treated himself: fair and square. Today, rooted in its rich heritage, J.C. Penney Company, Inc. is re-imagining every aspect of its business in order to reclaim its birthright and become Americaââ¬â¢s favorite store. . . . At every visit, customers will discover straightforward Fair and Square Pricing.â⬠17 The four-year plan involved several distinct, yet integrated elements that touched every part of the business and were designed to recreate a golden age department store that appealed to all Americans, across age, income, and geographic demographics. As Johnson explained, ââ¬Å"We are going to rethink every aspect of our business, boldly pursue change, and create long-term shareholder value, as we become Americaââ¬â¢s favorite store. Every initiative we pursue will be guided by our core value to treat customers as we would like to be treatedââ¬âfair and square.â⬠18 New Logo J.C. Penney had been tinkering with its brand logo, changing it three times in three years. In 2011, the company asked the public for help in redesigning the logo in a crowd-sourcing experiment. The winning design was submitted by a University of Cincinnati student and was unveiled with much fanfare via social media. In 2012, Johnson scrapped this design and hired an agency to redesign the logo once again. The new logo evoked the American flag with red, white, and blue colors and the letters ââ¬Å"jcpâ⬠in lower case font within a square that represented the new ââ¬Å"Fair and Squareâ⬠mantra. J.C. Penney, which many affectionately called ââ¬Å"Penneyââ¬â¢sâ⬠would now be known as ââ¬Å"jcp.â⬠(See Exhibit 6 for the new logo.) New Brand Spokesperson One of the most exciting and controversial developments of the plan was the announcement of comedian and talk show host Ellen DeGeneres as the new brand spokesperson. DeGeneres, who once worked at a J.C. Penney store as a teenager in Louisiana, appeared in television advertising, developed J.C. Penney themed skits for her popular talk show, and tweeted about the company on Twitter. Johnson proclaimed DeGeneres to be ââ¬Å"one of the most fun and vibrant people in entertainment today, with great warmth and a down-to-earth attitude. . . . Importantly, we share the same fundamental values as Ellen.â⬠19 Shortly after DeGeneresââ¬â¢ advertising debut, the conservative Christian group One Million Moms took offense, citing DeGeneresââ¬â¢ homosexuality asà problematic for the brandââ¬â¢s image and its traditional family shopper demographic. The group asked its members to boycott J.C. Penney and to call their local store manager to ask for DeGeneresââ¬â¢ removal as spokesperson. DeGeneres went on the offensive to defend her personal values and to reassert her relationship with her fans and with J.C. Penney, producing a witty, yet heartfelt response delivered on her talk show that quickly went viral on the social web. A firestorm erupted and played out on J.C. Penneyââ¬â¢s Facebook page, where both pro- and anti-gay posters pledged their support for and/or rejection of the retailer. J.C. Penney survived the controversy by standing firmly behind its choice of spokesperson. The protest event generated significant positive press for the company and Facebook feedback was more positive than negative. Riding the wave of publicity, J.C. Penney went on to feature two gay dads in a widely touted Fatherââ¬â¢s Day advertising campaign. New Store Design While the new logo and spokesperson were short-term fixes that could be executed quickly, Johnson knew from his experience at Apple that, to really make a difference, he had to make significant changes to the product offering, a longer term proposition. He embarked on a multi-year plan to re-energize and redesign J.C. Penneyââ¬â¢s product offering and its merchandising at retail. He began by forging new supplier relationships with top brands like Martha Stewart and hot designers like Nanette Lepore to create J.C. Penney-specific merchandise lines, a strategy reminiscent of Target. He then went to work to improve the quality of J.C. Penneyââ¬â¢s sagging and dated private label brands, Worthington, St. Johnââ¬â¢s Bay, The Original Arizona Jeans Co, and Stafford, to reinvigorate them and restore their brand integrity. These efforts could also build on J.C. Penneyââ¬â¢s recent purchase of the Liz Claiborne brands (which, among others, included Liz Claiborne branded apparel, L ucky Jeans, Kate Spade and Juicy Couture) and the ongoing opening of about 300 Sephora locations inside J.C. Penney stores, which offered a select set of Sephora beauty care products. 20 He envisioned the in-store retail environment as a series of interactive specialty ââ¬Å"Shops,â⬠along a visually engaging and vibrant ââ¬Å"Street,â⬠with a central ââ¬Å"Squareâ⬠that would serve as the social hub of the store. J.C. Penneyââ¬â¢s vast array ofà merchandise, currently hung on crowded racks and shelves, would be regrouped and merchandised in 80-100 ââ¬Å"stores-within-a-store,â⬠each meant to simulate the buying experience of a specialty shop. The first shop to appear was devoted to jeans and featured a denim bar, trained fit specialists, and Leviââ¬â¢s innovative Curve ID program that helped women find the right jeans for their body type (see Exhibit 7). Plans for future shops included Joe Fresh and Mango. The company planned to install two to three new shops each month, beginning in August 2012, over a four year period. Many of the shops were designed to pull in younger shoppers, a deficit in J.C. Penneyââ¬â¢s current customer base. The ââ¬Å"Streetâ⬠would consist of wider aisles with a fresh, clean look, more streamlined with less signage and bold, colorful, upscale graphics featuring the square from the new logo (see Exhibit 8). Each month would have its own unique personality and color-coded signage that changed the look of the store to freshen its appeal. Ten thousand square feet at the center of the store would be designated for the ââ¬Å"Town Square.â⬠In this area, J.C. Penney planned to offer complimentary services, such as gift wrapping, and special promotional events to create fun and excitement. During the summer of 2012, the company offered free hot dogs and ice cream, free ââ¬Å"Go USAâ⬠Olympic t-shirts during the Summer Olympics, and free back-to-school haircuts for school children. Johnson summarized his vision for the new environment, ââ¬Å"We are going to make the store a place people love to come-just to come. Weââ¬â¢ll transform the buying experience not unlike what we did at Apple.â⬠21 New Sales Structure To support the new retail environment, Johnson needed to re-energize J.C. Penneyââ¬â¢s sales force. His goal was to create a team of specialists who were product experts, much like Appleââ¬â¢s Geniuses. J.C. Penney sales clerks had always been paid commissions based on how much they sold. This system encouraged sales clerks to sell aggressively to customers. Johnson felt that this aggressive sales culture did not fit with the new ââ¬Å"Fair and Squareâ⬠positioning and set out to change it by eliminating all sales commissions. It was a controversial decision, especially among the sales employees, many of whom had just been through a wave of layoffs and were nervous about keeping their jobs. Johnson explained his rationale for the change, ââ¬Å"A lot of great retailers donââ¬â¢t use commissions. We never used them at Apple. . . . And I think itââ¬â¢s a better thing to do to pay people in advance for what you want them to do and let them look in the customersââ¬â¢ hearts and try to help them. . . . We think weââ¬â¢ve got a great way to do business for the middle class, where we really put a big bear hug around the middle class and help them look better and live better every day.â⬠22 But some employees expressed dissatisfaction, ââ¬Å"I must take offense at Ron Johnsonââ¬â¢s reason for eliminating commission. Ron Johnson should remember that J.C. Penney is not Target, we are better. When people come into our store they expect to be greeted, they expect someone to be available to help, they expect good service,â⬠said a sales associate. Another associate claimed, ââ¬Å"I lost about $250 per pay period and Mr. Johnson thinks this is FAIR and SQUARE. From all of J.C. Penneyââ¬â¢s little workers, this stinks.â⬠Another lamented, ââ¬Å"We long-term employees are heartbroken at what we see around us. Ron Johnson may have a grand plan, and it may work, but we feel like he is destroying ââ¬Ëusââ¬â¢ in the process of implementation. It has become an awful place to work, short-staffed to the point that we struggle to properly service what customers we do have.â⬠23 But without a doubt, the cornerstone of the change program was a new pricing scheme that many believed to be the riskiest part of the strategy. The New Pricing Strategy Looking at the numbers, Johnson believed that he needed to address the existing high-low pricing structure that had gotten out of control. J.C.à Penneyââ¬â¢s customers had become hooked on the deals; over the past ten years, the average discount to get customers to buy went from 38% to 60%24. ââ¬Å"At some point you, as a brand, just look desperate. J.C. Penney spent over $1 billion [on price promotion], and the customer didnââ¬â¢t even pay attention,â⬠he agonized.25 In his first report to shareholders, he spoke about the detrimental long term effects of excessive price promotions, ââ¬Å"Plagued by the ââ¬Ëgamesââ¬â¢ of the industry over the last several decades, retailers-including J.C. Penneybarraged customers with a constant stream of promotions that proved to be ineffective. Each time we participated in this pricing war, we were discounting our brand and eroding the trust and loyalty of our customers.â⬠The company announced its ââ¬Å"Fair and Squareâ⬠pricing plan in January 2012. The plan had three pricing tiers. First, the company reduced prices by an average of 40% to offer consumers an ââ¬Å"Every Day Fair and Squareâ⬠price. Second, every month the company ran a ââ¬Å"Month Long Values Eventâ⬠with special pricing on seasonal items, marked down an additional 20-29%, meant to coincide with events such as Back-to-School and Fatherââ¬â¢s Day. Third, every first and third Friday of each month (paydays for many working Americans) were designated ââ¬Å"Best Price Fridays,â⬠where J.C. Penney would offer special deals on items it was looking to liquidate, about 20% of the storeââ¬â¢s stock, at deals of about 1/3 off of the every day price. Each price point was supported by unique signage at retail, (see Exhibit 9). J.C. Penney eliminated its famous ââ¬Å"Doorbusterâ⬠sales, such as those that it traditionally held on Black Friday, the day after Tha nksgiving and the busiest shopping day of the year, that featured outrageously low prices onà over 500 items from 4:00 a.m. to 1:00 p.m. Exhibit 10 shows an example of the different price tiers. Importantly, J.C. Penney avoided using the words ââ¬Å"saleâ⬠and ââ¬Å"clearanceâ⬠in its messaging of the new program to consumers. Said Johnson, ââ¬Å"Sale is not in our vocabulary. . . . Every item in the store is priced to be its best price every day.â⬠27 The ââ¬Å"Fair and Squareâ⬠price was the only price listed on the price tag, moving J.C. Penney away from the practice of listing the manufacturerââ¬â¢s suggested retail price (MSRP) and the sale price, which was intended to show customers how much they were saving relative to somewhat fictitious list price. In the highly competitive world of retailing, nearly no one priced goods at the MSRP. Breaking with another retailing best-practice, J.C. Penney ended all of its ââ¬Å"Fair and Squareâ⬠prices with .00 instead of .99, rounding up to the nearest dollar. Johnson also instituted a no restrictions ââ¬Å"Happy Returnsâ⬠return policy, designed to take the hassle out of returning items, eve n without a receipt. In effect, the new plan combined elements of two traditional pricing strategies. The ââ¬Å"Every Day Fair and Squareâ⬠prices represented an everyday-low-price (EDLP) strategy, while the ââ¬Å"Month Long Valuesâ⬠and ââ¬Å"Best Price Fridaysâ⬠maintained some emphasis on high-low pricing. High-low pricing strategies are intended to allow retailers to use price discrimination to maximize the average price paid by customers who differ in their willingness to pay. Customers who are highly price sensitive wait for sale days to purchase, use coupons and rebates, scour the crowded clearance racks to find a bargain, and take advantage of retailerââ¬â¢s door buster specials on big shopping days like Black Friday. Customers who are less price sensitive buy when it is convenient for them, tend not to use coupons and rebates due to the time it takes to clip and organize them, and rarely join in on door buster specials or clearance sales. Thus, the retailer reaps higher non-sale prices from many of their purchases. However, given the predominance of high-low pricing strategies across retailers in todayââ¬â¢s marketplace, even less price-sensitive consumers had become savvy about waiting for sales to buy or comparing across retailers to find the store offering the best prices that week. Instantaneous price comparisons wereà getting easier, given the rise of mobile applications that allowed a consumer to scan a bar code on a product and find the lowest price for it at online retailers and nearby stores. Kohlââ¬â¢s was an aggressive high-low retailer, featuring small electronic signs on shelves throughout the store that displayed original prices and discounted prices. These signs allowed Kohlââ¬â¢s the flexibility to change prices instantly, to facilitate frequent, short-term sales. Marketing consultant Jonathan Salem Baskin offered his thoughts on the high-low practice retailers engage in, ââ¬Å"When no price is ââ¬Ëtheââ¬â¢ price for an item, it means that instead retailers engage customers in a constant cat-andmouse game in pursuit of the truth. No individual store can own sale pricing; each simply participates in a round-robin of discounted offers that its competitors have and/or will again match.â⬠28 Johnson felt that todayââ¬â¢s retail customer was savvy, ââ¬Å"The customer knows the right price. To think you can fool a customer is kind of crazy.â⬠2 EDLP pricing strategies, such as that offered by Walmart, promise consumers that they will pay the same, low price every day. This frees customers from waiting for sale periods to purchase, and eliminates the need for retailers to offer coupons to drive purchase or to engage in constant advertising of price promotions via weekly newspaper circulars. EDLP is designed to make customers feel comfortable purchasing at the retailer without worrying that they could be getting a better deal somewhere else or at another time. In general, most department store retailers used high/low pricing strategies. Macyââ¬â¢s and Sears had flirted with EDLP pricing in the past; but both had largely abandoned it once they realized how addicted department store customers were to sales, coupons, and other discount programs. Althoughà Macyââ¬â¢s still offered a limited set of items at an ââ¬Å"everyday valueâ⬠price, it heavily supplemented this practice with aggressive coupons and frequent sales events for the majority of the goods it carried. Macyââ¬â¢s customer Marietta Landon summarized the promotion addicted retail climate, ââ¬Å"Especially Macyââ¬â¢sââ¬âthey make every weekend a sale with saving passes and advertising galore.â⬠30 The new pricing strategy was a big shift for J.C. Penney, a company known and loved for its JCP Cash coupons distributed to customers via direct mail and email, its RedZone Clearance aisles, and its weekly circulars advertising that weekââ¬â¢s price specials. The ââ¬Å"Fair and Squareâ⬠pricing program would eliminate all coupons and weekly circulars; instead the company would distribute a high quality, editorial content-heavy glossy magazine each month to highlight its Monthly Values. The 96page magazine was as much a branding vehicle as it was a promotional one. $80 million in promotional funding would support each Monthly Value event. J.C. Penney now promised its customers that they would not have to ââ¬Å"jump through hoops to get a good priceâ⬠. Johnson hailed the strategy for its simplicity and transparency and the way it respected customers, ââ¬Å"People are disgusted with the lack of integrity on pricing,â⬠31 adding that ââ¬Å"We want shoppers to shop on their terms, not ours.â⬠Johnson intimated that ââ¬Å"By setting our store monthly and maintaining our best prices for an entire month, we feel confident that customers will love shopping when it is convenient for them, rather than when it is expedient for us.â⬠32 Michael Francis, J.C. Penneyââ¬â¢s new president, was excited about the new pricing moves, ââ¬Å"We are redefining the J.C. Penney brand so we become a store for all Americans, by offering an experience they cannot get anywhere else. This will start by freeing consumers from the barrage of promotions and undifferentiated shopping experiences they have become used to and replacing it with something entirely fresh and new that is evident in every aspect of our store.â⬠33 He added, ââ¬Å"It will be a breath of much-needed fresh air and give [customers] reasons to visit J.C. Penney more often than ever before. Our objective is to make our customers love to shop again.â⬠34 Francis was recruited by Johnson from Target and offered a signing bonus of $12 millionà and a total compensation package worth $44.7 million. He was charged with managing the marketing and merchandising efforts. Reactions to ââ¬Å"Fair and Squareâ⬠Pricingà Industry observers could not contain their strong opinions on the new pricing strategy. Some called the move ââ¬Å"refreshing, daring and probably exactly what the retailer needs,â⬠noting that ââ¬Å"itââ¬â¢s a shocking move for any retailer, let alone a department store where high-low pricing and promotions have long been the norm.â⬠35 But others were far more skeptical. Pricing consultant Rafi Mohammed proclaimed, ââ¬Å"J.C. Penney lacks the differentiation to make this pricing strategy successful. . . . When selling a relatively undifferentiated product, the only lever to generate higher sales is discounts. Even worse, if competitors drop prices on comparable products, J.C. Penneyââ¬â¢s hands are tied-it is a sitting duck that canââ¬â¢t respond.â⬠36 Mohammed also noted, ââ¬Å"J.C. Penneyââ¬â¢s Every Day prices will not be as low as the biggest discounts that it once offered. Instead, its pitch to customers is why play the ââ¬Å"wait for the rock-bottom priceâ⬠game when Penney offers ââ¬Å"pretty goodâ⬠prices every day?â⬠37 Ignoring the skeptics, Johnson was committed to his new pricing plan, rolling it out across all stores on February 1st, after deciding not to conduct market research to test its appeal with customers, ââ¬Å"We debated whether there was a way to test. . . . We would have needed everyone to run the old business model and would have had to add new people to run a test in 10 percent of our stores. . . . We knew the customer would love the new strategy. We decided to get on with our future.â⬠38 Based on his experience at Apple, Johnson also believed thatà customers didnââ¬â¢t always know what they wanted; it was up to companies to lead the way, ââ¬Å"You canââ¬â¢t follow the customer. Youââ¬â¢ve got to lead your customersââ¬âanticipate their needs and meet those needs, even before they know what they want.â⬠39 A lot was riding on the decision. COO Mike Kramer explained, ââ¬Å"We are fundamentally reimagining every aspect of our business and we fully expect the bold and strategic changes we are making to our operations will result in improved profitability. This should enable us to fund the transformation of J.C. Penneyââ¬â¢s store experience, while at the same time returning value to shareholders with steady earnings growth.â⬠40 Communicating ââ¬Å"Fair and Squareâ⬠DeGeneres was featured in a new advertising campaign to usher in the new ââ¬Å"Fair and Squareâ⬠positioning. Bearing the tagline ââ¬Å"Enough. Is. Enough,â⬠the campaign encouraged consumers to revolt against complex pricing structures, never-ending sales, an overabundance of direct mail circulars and coupons cluttering their mailboxes, and the hassles of returning unwanted products without a receipt. In the ads, DeGeneres travels back in time to ancient Rome, Edwardian England, and the Wild West to learn if todayââ¬â¢s confusing price environment was always the norm. She encourages customers to reject the crazy price environment. The creative campaign was witty and contemporary; many found it reminiscent of Targetââ¬â¢s award-winning advertising. It was quite a departure from J.C. Penneyââ¬â¢s previous campaigns that were more typical of department store messaging. Launched during the Academy Awards broadcast, the ads appeared to be a hit with consumers. Ace Metrix reported that the ads scored well above average on persuasion and watchability metrics and achieved a personal best score for J.C. Penney.41 Initial Results In the first three months following the launch, 67% of products sold at J.C. Penney were purchased at the ââ¬Å"Fair and Squareâ⬠price, the highest price the retailer listed. Johnson could not hold back his satisfaction, ââ¬Å"This is profound. People are now buying at the first price, [the] right price. Thatââ¬â¢s the dream of every retailer.â⬠42 However, trouble was looming on the horizon. Through mid-March, mothers, a critically important target market for most department stores, steadily scored J.C. Penney lower on valueperception scores. These women, suddenly not receiving coupons and not seeing the weekly price promotions in the circulars, were downgrading their opinion of whether J.C. Penney offered good value for the money.43 This was despite the fact that J.C. Penneyââ¬â¢s prices during the time period were actually quite competitive. A Deutsche Bank analyst report showed that for a random basket of 50 identical items, J.C. Penney was 9% cheaper than Macyââ¬â¢s, and 26% cheaper than Kohlââ¬â¢s. Consumer research firm BIGInsight reported negativity among adults 18+ for whether J.C. Penneyââ¬â¢s advertising campaign was ââ¬Å"Hot or Not?â⬠and showed Macyââ¬â¢s gaining ground on J.C. Penney in womenââ¬â¢s apparel shopping trips following the launch (See Exhibit 11). Morgan Stanleyââ¬â¢s Michelle Clark reported consumer survey results revealing that ââ¬Å"Shoppers think that the J.C. Penney of old actually offered better value than the ââ¬Å"fair and squareâ⬠model introduced a few months ago. Of the consumers who had been inside a J.C. Penney store since February, more cited higher prices (rather than lower) at the department store. In fact, only 16% of shoppers associated ââ¬Å"Best Pricesâ⬠with JCP. Furthermore, customers cited that bargains were harder to find and fewer aisles with deals were evident (see Exhibit 12).45 Loyal J.C. Penney customers were moving away from the retailer. One shopper, Wendy Ruud, complained that she was no longer receiving coupons from J.C. Penney and was shopping more frequently at Target and Walmart, ââ¬Å"The closest J.C. Penney is about a half hour away from me. If I donââ¬â¢t get a specialà discount, itââ¬â¢s not worth the trip,â⬠she said.46 Another shopper e-mailed the Huffington Post saying, ââ¬Å"They are catering to the younger shopper, and it isnââ¬â¢t the younger shopper that kept them afloat.â⬠47 A third who considered herself ââ¬Å"frumpy and proud,â⬠commented, ââ¬Å"Heââ¬â¢s working hard to ââ¬Ëde-frumpââ¬â¢ the store without considering that many if not most of its customers might have shopped there precisely because they like the more conservative frumpy look.â⬠48 These early indicators played out in J.C. Penneyââ¬â¢s first earnings report following the launch of the new plan. Johnson had to announce a significant earnings loss ($163 million) based on plummeting sales revenues (-19% overall, with e-commerce sales dropping 28%), gross margin compression (from 40.5% to 37.6%), and decreasing customer conversion. Johnson asked investors to be patient, calling the first quarter sales drop ââ¬Å"the price weââ¬â¢re paying to get integrity back.â⬠49 He held fast to his convictions, ââ¬Å"We had to make the bold step. Itââ¬â¢s one big year we have to go through. Itââ¬â¢s really hard but weââ¬â¢ll get through it.â⬠50 Investors showed no patience, sending the companyââ¬â¢s stock down 20%, the biggest single day drop in over four decades.51 The critics did not waste time to pile on Johnson. Time columnist Brad Tuttle wrote, JC Penneyââ¬â¢s message seems to be one that some shoppers donââ¬â¢t want to hear. They like playing games and hunting for deals, and the markdown from the original price is how they keep score. By eliminating coupons and most ââ¬Å"sales,â⬠JC Penney has been saying it doesnââ¬â¢t want to play games anymore. That sounds wonderful, but among certain shoppers, itââ¬â¢s the equivalent of grabbing the ball and taking it home. No more games, no more fun-and not much reason to visit JC Penney on a regular basis anymore. If, for the most part, a storeââ¬â¢s prices are going to remain the same tomorrow, and next week, and the month after that, thereââ¬â¢s not much incentive to browse the aisles for special deals today.52 A Forbes columnist concurred, ââ¬Å"By taking away the weekly sales customers loved, Johnson abandoned his core JCP shopping enthusiasts. In effect, signaling to the core JCP enthusiastsshoppers who have sustained J.C. Penney through its years of retail muddling, that they no longer mattered. He confused them, and he pissed them off.â⬠53 The Motley Fool sarcasticallyà quipped, ââ¬Å"The silver lining in J.C. Penneyââ¬â¢s awful report is that Sears [struggling with its own dismal results] has someone it can laugh at now.â⬠54 Macyââ¬â¢s CFO Karen Hoguet was crowing that her company was benefiting from J.C. Penneyââ¬â¢s missteps, reporting that sales in Macyââ¬â¢s stores that shared a mall with J.C. Penney were up significantly since the changes.55 And J.C. Penneyââ¬â¢s apparel suppliers were becoming anxious, as their sales dropped precipitously, some as much as 70% over the prior year. One prominent supplier indicated that he was increasing his business with Kohlââ¬â¢s to make up for the shortfalls at J.C. Penney.56 These developments were sobering for Johnson yet he remained unfazed, ââ¬Å"Itââ¬â¢s been tougher than we anticipatedâ⬠¦You know, we expected to be down. We are down a little more than we thought, but not enough to change the strategyâ⬠¦Weââ¬â¢re treating this company as a startupâ⬠¦Weââ¬â¢re inventing a whole new model to do businessâ⬠¦It is a one year transition thatââ¬â¢s part of a multi-year transformation. But once we get to one year of de-promoting or repurchasing our integrity, I fully expect us to grow. And so weââ¬â¢ve just got to get through that year. And weââ¬â¢ll get through it.â⬠57 Speaking at Fortune magazineââ¬â¢s Brainstorm Tech conference in July, Johnson reiterated his support for the new pricing strategy, claiming that his board was ââ¬Å"totally supportiveâ⬠. When asked if he had a contingency plan whereby the company would revert back to high/low pricing, Johnson swore it was not in the cards, ââ¬Å"It wonââ¬â¢t happen while Iââ¬â¢m here because I know itââ¬â¢s not the right thing to do. And I know this is what connects completely with our own unique heritage. And every longstanding company has a DNA in its core thatà typically goes back to its founder. And when you reconnect with that, thatââ¬â¢s when good things happen. Thatââ¬â¢s what Wal-Mart has had to do. And itââ¬â¢s really led to great success. Thatââ¬â¢s what Apple had to do when Steve came back. Thatââ¬â¢s what weââ¬â¢re going to do.â⬠58 Making Some Adjustments As J.C. Penney management tried to decipher the disappointing results, much of the blame was put on the marketing execution and on customersââ¬â¢ stubborn reliance on price promotions. Mike Kramer, J.C. Penneyââ¬â¢s new chief operating officer expressed his frustration, ââ¬Å"Coupons, that drug. We did not realize how deep some of our customers were into this. . . . We have got to wean them off this and educate our consumers.â⬠59 Johnson blamed the marketing execution, claiming that it failed to clearly communicate the new pricing strategy, ââ¬Å"Our execution wasnââ¬â¢t what we needed. Our pricing is kind of confusing. Our marketing kind of overreached [Now] the most important thing is to educate consumers on the price changes and make sure the core customer understands J.C. Penney still has products they love, at exceptional value, every day.â⬠60 Francis took the fall for the poor earnings, abruptly leaving the company a mere eight months after he started as pr esident. Following Francisââ¬â¢ departure, Johnson took responsibility for marketing and merchandising, believing that customers just didnââ¬â¢t understand the story behind ââ¬Å"Fair and Square.â⬠He tweaked the marketing plan, adding five additional ââ¬Å"Best Price Fridaysâ⬠to the calendar, including the important Fridays anchoring Memorial Day Weekend and Black Friday. The advertising creative was changed to incorporate a harder-hitting ââ¬Å"Do the Mathâ⬠positioning (See Exhibit 13 for an example). In June, J.C. Penney reintroduced the ââ¬Å"Sâ⬠word ââ¬Å"saleâ⬠into its advertising to help clarify that its Best Price Friday deals actually extended through the weekend until all inventory was sold. Under pressure, Johnson speculated what his old mentor, Steve Jobs, who passed away in October 2011, would have advised, ââ¬Å"I think Steveââ¬â¢s advice would be donââ¬â¢t worry about what others say. Trust your instincts. Do the right thingâ⬠¦Stay the course. But he would also say the essence is in the simplicity. And so he would have liked where we are going on pricing, but he would have said ââ¬ËYouââ¬â¢ve got to clean it up. Youââ¬â¢ve got to be more directââ¬â¢.â⬠61 Johnson buckled down, ââ¬Å"What you canââ¬â¢t do is chicken out.à If you had looked at the data on the Genius Bar after a year and a half, we should have taken it out of the store. But it was something I believed in with every bone in my body.â⬠62 He continued, ââ¬Å"The world moves by innovators and innovators have to have the courage to imagine something that hasnââ¬â¢t been done before and the conviction to see it throughâ⬠¦It is really hard. It takes a lot of courage. Youââ¬â¢ve got to be able to have a few arrows shot in your back.
Sunday, July 21, 2019
Putative S-Adenosyl Methionine Dependent Ironââ¬Sulfur
Putative S-Adenosyl Methionine Dependent Ironââ¬âSulfur Identification and Characterization of a Putative S-Adenosyl Methionine dependent Ironââ¬âSulfur containing protein fromà Methanococcus Jannaschii Qi Liu Research Proposal Dr. MishtuDey Dr. M. Lei Geng Dr. Leonard R. MacGillivray Dr. Amnon Kohen Dr. Daniel Quinn Introduction Biological methane formation is a microbial process that is catalyzed by microbes called methanogens, which belong to the third kingdom of life, the Archaea. Methane is formed at the final catalytic step by methyl-coenzyme M reductase (MCR), in which coenzyme B (CoBSH, N-7-mercaptoheptanoylthreonine) donates two electrons to reduce methyl-coenzyme M. MCR is a 300kDa enzyme, which is composed of three different subunits in an à ±2à ²2à ³2 arrangement and contains 2 mol of the nickel tetrapyrrole coenzyme F430, which are buried deeply within the protein complex[1]. There are five modified amino acids were found out on the à ±-subunit and near the active site of MCR from methanothermobacter marburgensis based on the X-ray crystallographic studies. They are 1-N-methylhistitine (Hisà ±400), 5-(S)-methylarginine (Argà ±271), 2-(S)-methylglutamine (Gluà ±400), S-methylcysteine (Cysà ±452), where the side chains are methylated and one thioglycine (Glyà ±445) forming a thiopeptide bo nd (Figure 1). Since the DNA sequence of the encoding MCR gene shows no unsusal condons or unusual codon usages at the positions in which the five modified amino acids were found, the modifications are introduced after translation [1]. According to vivo labeling experiments with L-(methyl-D3)-methionine, people found that the methyl groups from the modified amino acids are introduced biosynthetically from the methyl group of methionine by specific S-adenosylmethionine (SAM) dependent Figure1. Post Translational Modifications in MCRà enzymes. These methyl translational modifications are catalyzed by protein methylases that specifically recognize the amino acid sequences up and downstream of amino acid to be methylated. The genome of methanogens has many open reading frame predicted to be putative methyltransferases, which also agrees with the proposal that there are four different SAM-dependent protein methylases are involved in the post translation modification. A search of six kn own methanogenic genomes led to the identification of conserved open reading frame around the MCR gene cluster. Some methangenic archaea contain two MCR isoenzymes, designated MCR1 and MCR2. This conserved hypothetical protein is found around MCR1 in Methanococcus jannaschii and Methanobacterium thermobacter. The open reading frame from Methanococcus jannaschii, MJ0841 is annotated as a conserved hypothetical protein, which is found to be related to the radical SAM enzyme superfamily. The signature motif of SAM radical enzymes is three cysteine motif ââ¬Å"CX3CX2Câ⬠(Figure 2), multiple sequence alignment of the putative gene from methanogens show the conserved CXGFCXXC, which is known to coordinate to [4Fe-4S] cluster. (Figure 3) Figure 2. Multiple Sequence Alignment of MJ0841 Homologues from Different Methanogens Figure 3. [4Feââ¬â4S] cluster coordinated by three-cysteine motif CxxxCxxC. The fourth iron of the cluster interacts with a bound SAM. Specific Aims It is interesting and important to determine the function of this hypothetical protein. We speculate that this hypothetical protein, MJ0841, could be a possible candidate responsible for the post-translation modification of the methylated amino acids, or, is involved in the formation of the thioglycine in MCR. Research Plan, Results and Discussion Expression and Purification of MJ0841 MJ0841, a 1248bp gene, was cloned into pET28a vector. The resulting plasmid was transformed in E.coli BL21(DE3) cells for gene expression. In order to increase the iron content, MJ0841 was also co-expressed with PDB1282. Overnight cell culture grown at 37oC in Luria-Bertani (LB) medium containing both kanamycin (50ug/ml) and ampicillin (100ug/ml) was inoculated, in a 100-fold dilution, into Terrific Broth (TB) media aerobically at 37oC. FeCl3 was also added to a final concentration of 100uM to be as the iron content for iron-sulfur cluster during the growth when OD600 was around 0.3. Protein expression was then induced at OD600 of 0.6 to 0.7 with addition of Isopropyl à ²-D-1-thiogalactopyranoside (IPTG) to final concentration of 0.5mM. After overnight incubation at 37oC around 18hours, the cells were harvested by centrifugation at 5000rpm for 30mins at 4oC, and stored at -80oC. The following procedures were all carried out in oxygen free environment at 20oC. Purification was conducted anaerobically at oxygen level always below 2.0ppm in anaerobic chamber. 23g Cell were moved into anaerobic chamber and resuspended in 120mL lysis buffer (50mM tris-HCl, 300mM NaCl, 5% glycerol pH 7.5), and Phenylmethanesulfonyl fluoride (PMSF) 1mM final concentration, 3 tablets of protease inhibitor, 2-mercaptomethanol 10mM) for 15mins. The cells were lysed by sonication for 15mins followed by centrifugation at 30,000 rpm for 40mins at 4oC to remove the cell debris. The supernatant was applied to a packed 15mL Ni-NTA resin column equilibrated with lysis buffer. The column was then washed with 5 column volume of wash buffer (50mM tris-HCl, 300mM NaCl, 10mM IMD, 5% glycerol, pH 7.5). The brownish protein was eluted by gradient elution with 5 column volume of wash buffer and 5 column volume elution buffer (50mM tris-HCl, 300mM NaCl, 200mM IMD, 5% glycerol, pH 7.5). SDS-PAGE was applied to analyze the desired clean protein fractions, which were then combined an d set for overnight dialysis with dialysis buffer (50mM tris-HCl, pH 7.5, 5% glycerol) with slow stirring. The pooled fractions were concentrated using an Amicon centrifugal filter with a 30kDa molecular weight cut off (MWCO). The collected protein was further purified with 20mL packed Q-sepharose column equilibrated with lysis buffer (50mM tris-HCl, 5% glycerol, pH 7.5). The column was then washed with 5 column volume of wash buffer (50mM tris-HCl, 200mM NaCl, 5% glycerol, pH 7.5). The brownish protein was eluted by gradient elution with 5 column volume of wash buffer and 5 column volume elution buffer (50mM tris-HCl, 700mM NaCl, 5% glycerol, pH 7.5). SDS-PAGE was applied to analyze the desired clean protein fractions, which were then combined for reconstitution (Figure 4). Figure 4. SDS-PAGE gel electrophoresis analysis of MJ0841 purification fractions Reconstitution of the [4Fe-4S] Cluster of MJ0841 in Vitro The above apo-protein (16uM, 40ml) was incubated with final concentration of 5mM DTT for 1h with slow stirring at room temperature. Then, cystein was added into the above solution by dropwise to reach 10 molar folds excess of protein. After 30mins incubation, 10 molar excess of Fe(NH4)2(SO4)2 was added slowly to provide enough iron content for iron sulfur cluster and incubated for 30mins. The resulting solution was incubated with 10 molar excess of Na2S finally, and the brownish protein solution changed to dark brown after adding Na2S. The above final protein solution was kept in 4oC overnight around 14 hours for building up enough [4Fe-4S] clusters. In order to remove the unbounded iron and sulfur, the overnight reconstituted protein was concentrated to 2.5 ââ¬â 3ml of final volume and loaded onto a 5mL PT10 column equilibrated with lysis buffer (50mM tris-HCl, 5% glycerol, pH 7.5), and the final pure protein was combined. Reduction of [4Fe-4S] Cluster UV-vis spectroscopy was applied here for detecting the reduction of [4Fe-4S] cluster. A characteristic peak for [4Fe-4S] cluster was shown up near 412nm before reducing. Sodium Dithionite was used as the reducing agent and the stock solution was prepared freshly right before adding into the protein. 100 equivalents of sodium dithionite were mixed with concentrated protein, and the peak at 412nm was reduced (Figure 5). According to the results from UV-vis, the 4Fe-4S cluster was built up by reconstitution, also the [4Fe-4S]2+ was reduced to [4Fe-4S]+ by the reduction of sodium dithionite. Figure 5. UV-vis spectra of purified reconsituted MJ0841 Figure 6. EPR spectrum of as-isolated MJ0841 (blue trace) and purified reconstituted MJ0841 originating from a [3Fe-4S]+ cluster. reduced with 100 equiv sodium dithionite (red trace) EPR samples preparation and spectral collection EPR spectrums of as-isolated, oxidized and reduced form of MJ0841 are detected at 10K. All samples are prepared anaerobically. As-isolated protein was prepared with the protein without reconstitution, and exhibits a strong isotropic EPR signal, which is centered at g=2.01 same as the g=2.01 signal of the [3Fe-4S]+ cluster form (Figure 6). The oxidized form protein containing [4Fe-4S]2+ was prepared by injecting 200ul concentrated protein purified through PD10 column into EPR tube, and it normally shows silent EPR signal (Figure 7). Reduced form of as-isolated protein was performed by mixing with 100 equivalents of sodium dithionite with 200ul concentrated protein, which gives the reduction form of [4Fe-4S]+ cluster and shows the characteristic EPR signal with g factors of g=2.03 and g=1.92 (Figure 8). Figure 7. EPR spectrum of oxidized form of MJ0841. Figure 8. EPR spectrum of reduced form of MJ0841. SAM cleavage activity of MJ0841 The characteristic reaction for detecting radical SAM enzymes is reductive cleavage of SAM into S-adenosylhomocysteine (SAH) and 5ââ¬â¢-deoxyadenosyl radical (5ââ¬â¢dAdo). Assays were conducted under strict anaerobic conditions. The reaction assay contains the following: 50mM Tris-HCl, 5mM DTT, 5Mm sodium dithionite, 0.5mM SAM. Reactions were initiated by addition of SAM and carried out at 20oC for 20 hours. The control reaction was run under the same conditions as the above assay, but without presence of protein. Trifluoroacetic acid (TFA) (final concentration 5% v/v) was added to quench the reactions, which then were identified by HPLC analysis. HPLC analysis of SAM cleavage assay products After quenching by TFA, the reaction mixture was centrifuged and the supernatant was applied into HPLC analysis. 10ul of assay mixture was injected into C18 column, which had been pre-equilibrated with equilibrium buffer (40mM ammonium acetate, pH=6.2). Then the column was washed with a linear gradient from 0-50% acetonitrile for 30mins at room temperature to detect SAH and 5ââ¬â¢dAdo. The UV-detector was set at wavelength 258nm, and the standard samples, SAM, SAH, and 5ââ¬â¢dAdo, were run with the same condition as SAM cleavage reaction products. According to the retention time comparison between standard samples and products, formation of SAH and 5ââ¬â¢dAdo were all detected via HPLC analysis when enzyme was present. In the absence of enzyme MJ0841, SAM was not consumed at all and there were no any products peak formed, which confirmed the SAM was cleaved by enzyme. The dark blue, red, light blue traces show the relative intensities of 5ââ¬â¢dAdo, SAH, and SAM standards. The green trace shows the assay with the use of reconstituted MJ0841, and the SAH and 5ââ¬â¢dAdo were both observed. The purple trace shows the control assay without MJ0841, and there was not any of 5ââ¬â¢dAdo formed (Figure 9). Figure 9. HPLC analysis of the SAM cleavage assays Conclusions Future Work Initial results seem to show the [4Fe-4S] cluster and the enzyme activity. Since SAM was cleaved enzymatically, the products will be detected by mass spectroscopy to confirm the formation SAH and 5ââ¬â¢dAdo. Furthermore, probable substrates of MJ0841 will be prepared, which should contain the amino acids that would be modified. The activity assays with substrates will be examined to detect the desired methylation reaction on substrates.
Saturday, July 20, 2019
The role of women in society and the church
The role of women in society and the church Introduction I am going to discuss the role of women in the church and contentious issues, particularly when the passages such as 1 Timothy 2:11-14, and 1 Corinthians 13:34 talk about women should be silent in church. And slightly discuss the women in the society. The Role of Women in the Society Women in the modern society Women today are coming forth in modern social trends. This feminism may appear too extreme and rather threatening to the male ego, yet the role of women in current society has drastically changed. Perhaps there is some explanation for their actions. Women are now regularly performing duties that were traditionally assigned to males. They are attempting to fight the continuing stereotype established during the earlier part of the century. The pretty image of the perfect fifties wife is rapidly evolving into a more suitable projection to accommodate their new role in todays society. More than two generations ago, a girl was expected to abandon her academic pursuits and assume the role of a housewife. She would completely devote herself to the preservation of the female-oriented position of homemaker. As time has progressed, fluctuations in the economy have made it necessary for a woman to obtain a career in order to fit in the society. Strange as it may seem, the family roles have not changed much in todays society. While girls and women (not only Polish) have more opportunities in home based employment, the traditional roles of women are still quite evident. They are still the caring provider and nurturer of young infants and children, the comforter for the crying child and the feminine presence of the household. While the husbands role has diversified into more household chores and the cooking and cleaning up responsibilities, they are still considered the head of the household. In the absence of the man, can the woman really be like the man? I think yes. There are women who are capable in finance, Do-It-Yourself fixtures and fittings, even being a capable single parent and bread winner. In this respect, women will have to be like the man. Of course, the woman cannot match the physical strength of the man. The other aspect of thinking like the man is a definite no. This is through experience. Women generally look at the details and forget about the overall picture. They have the capability of juggling more than one issue at a time unlike the man. And she is more tempered to her feelings. And because of this, the focus is not there to make a sound judgment. Men on the other hand, are more focus and look at one thing at a time. They are less prone to feelings and therefore they are better at seeing the overall picture. I think for this matter, a man balances up the woman and vice versa. If we look at the example of a family with the wife who tries to be like the man, we have a situation where the roles are different now, because of the presence of the man. Man, no matter how gifted or ungifted have a certain ability to say that he is the man in the house. And for the wife to usurp his position there will be certain unrest in the overall relationship. This may not be currently evident but it will become an issue at a later stage. Subtle hints are always there and if the wife chooses not to suspect or confront the situation, it will lead to a crisis. Men and Women in Worship. I will begin by what Paul stated concerning men and women in worship that men should adopt a respectful attitude as they pray and also women should pray with an altitude of respect, just like men. It might be a different thing in that church which Paul was addressing. Women face particular problem, in that it is easy for them to become preoccupied with their appearance and with looks that take time to achieve. Itââ¬â¢s true that Descent and Honorable behave matters far more to God than jewels and expensive clothes. Beside if Christian women who posses Gods worship are too concerned about their external appearance they will forget the essential and will have neither the time of doing good deed. Itââ¬â¢s important to emphasize that Paul was not forbidding beautiful jewelry or lovely hair styles to all Christian women of all time was warning against becoming so attached to clothes and fashions and forgets the most important thing that is pleasing God by doing good deeds. Paul Advice Women to Be Quite During Teachings This advice has raised much debate among Christians in Africa, especially since the raise of feminist theology that asserts among other things, the right of women express themselves. Certain versions of the Bible have tried to get to get around the problem of translating 2:11 in a more acceptable way while still being true in the spirit of the text. The massage translates this as i dont let women to take over and tell men what to do. This translation assume that Paul give this advice because women were talking positions of authority and even of dominance over men, which was unacceptable in the culture of that day. The requirement for silence in association with teaching may also indicate that the women were talkative during time of teaching and worship. Paul advice women to be silence not because of inferior, but as a sign of their submission to men and of acknowledgment of mens authority. They will then be obeying the Oder that God established at creation. In applying this instruction in Kenya today, we need to think carefully about how women express their submission to authorities and to men. THE ROLES OF WOMEN IN CHURCH IN KENYA TODAY The role of women in the church is a contentious issue, particularly when passage 1 Timothy 2:11-14 is a regarded as laying down absolute rather than general principles within a particular culture. The focus should fall on the biblical call for all human beings to decern what is the will of God in their live ( roman 12:2) Jesus radical mission of transformation for liberation. Means that women and men are equally called and empowered to participate in the same mission in the church. What women and men do depends on our obedience to the guidance and empowerment of the Holy spirit. Because of the entrenched culture and sexist attitude and practices, and the male-dominated leadership in many of the churches especially in the Maasai land, women in Kenya church have critical and prophetic role to play in stirring waters and speaking the truth by asserting their God given humanity and gift not for their own sake of integrity of the gospel. many women in Kenya church have continue to claim their full potential and have taken leading roles. like the Samaritan woman ( John 4:1-42) when women in the Kenya church drink the water offered by Jesus, they go out to witness and spread the word of truth in their homes, villages, communities and churches with determination, boldness, courage and humility. for example the like of Teresia Wairimu, Esther Obasike, Bishop Magret Wajiru and many of great women of God which some have founded churches , preached, taught christian theology seminary schools and translated scripture into different languages in Kenya. Where the church have listen the voice of the Holy Spirit , they have realize that scripture grant women a place in all ministries of the church. And women have been rightly ordeined to serve in all ministries of the church. Conclusion A careful examination of the Bible will yield an understanding of Godââ¬â¢s intent for women and men to be full and equal partners to serve and worship Him. Perhaps the easiest point to make and understand is that there are many biblical examples of women leaders. The following women were divinely called to lead, were affirmed by their religious communities, and were specifically recorded in scripture as part of Godââ¬â¢s revealed truth. A few examples: Miriam ââ¬â prophet; played major role in Exodus; led worship singing and dancing. Deborah ââ¬â prophet; judge; led Israeli troops into battle with Barak Lydia ââ¬â business woman who became a central leader in the Philippian Church Priscilla ââ¬â helped Paul establish churches at Corinth and Ephesus; corrected Apolloââ¬â¢s preaching Phoebe ââ¬â deacon; commended by Paul for her service to the church If by design God created women not to be leaders, then he would be going against his own order and intent to place women in leadership; but God does not violate His own design. In the beginning humans were created male and female in equality and partnership reflecting the image of God both individually and together as a plural unit. The sequence of creation does not signify importance or superiority. Adam is not superior because he is first; Eve is not the new and improved model. Instead, Eve is called Adamââ¬â¢s helper. The word helper, however, is not used as an indication of rank as in ââ¬Å"a boss and an assistant.â⬠The Hebrew word is ââ¬Ëezer and is also used to describe God by Moses in Exodus 18:4 when he says, ââ¬Å"My fatherââ¬â¢s God was my helper: he saved me from the sword of Pharaoh.â⬠Since God is called a helper also, we need to maintain the original elevated biblical definition of this word, and not submit to a culturally lowered interpretation in our understanding of Godââ¬â¢s creation design Adam and Eve are both punished for disobeying God. One of the consequences of Eveââ¬â¢s sin was a distorted relationship with Adam. However, God in his mercy promised a reversal of the fall and restored divine and human relations through Jesus Christ. Restoration in Salvation: Salvation through Christ reverses or removes the punishment of judgment. Individuals are restored to a relationship with God. While we live in a world where the effects of sin are still playing out, believers are now members of the Kingdom of God where equality in him is restored. But even before Christ, faithful women were already filling places of leadership indicating that oneââ¬â¢s relationship with God has an impact that brings about a change on the judgment meted out at the fall. Refrence list 1 The Bible 2 http://www.gci.org/church/ministry/women6b 4.Sanley J. Grenz (1950-2005) :A Biblical Theology of Women in Ministry. Wolfhart Pannenberg publisher. 1
Jews Demand Holocaust Reparations :: essays research papers
American Dissident Voices Broadcast of April 7, 2001 As Ye Sow . . . . By Dr. William Pierce Hello! First, I want to admit to an error I made in last week's broadcast. I attributed to the circus impresario P.T. Barnum the statement that no one ever lost a nickel by underestimating the intelligence of the American public. At least two alert listeners have since pointed out to me that the statement actually was made by the journalist and writer H.L. Mencken. The statement for which P.T. Barnum should be remembered is that a sucker is born every minute. Last week we talked about the rewards of increasing "diversity" in America. The primary reward is the growth in alienation as young Americans fail to develop a healthy sense of racial identity and fail to find satisfactory role models. Diversity goes up, and homogeneity simultaneously goes down. And as homogeneity goes, so goes the individual's sense of community, of belonging, of identity. And we also talked about many of the side benefits of diversity: rising suicide rates, a greatly increased incidence of neuroses and psychopathologies, more schoolyard shootings, a growing pregnancy rate among unwed White teenagers, the confusion and loss of values, lowered academic standards, the trashing of the history and literature curricula in our universities, and a number of other consequences of the diversity being visited upon us by the government and the Jewish media. And America is not the only nation ruined by the diversity-mongers. If you've been paying attention to the news of what's happening in the Balkans, you will have noticed that the bloodshed and misery begun there by the determination of Madeleine Albright and the Clinton government to ram multiculturalism down the throats of the Serbs is still spreading. The killing going on in Macedonia now is a direct consequence of the Clinton government's bombing of Belgrade in order to force the Serbs to accept the multiculturalization of Serbia's Kosovo province. If you remember, the excuse given by Madeleine Albright and her kosher crew was that the wicked Serbs were mistreating the Albanian minority in Kosovo, who merely wanted to live in multicultural bliss with the Serbs. Well, of course, the Albanians wanted no such thing. They wanted exactly what the Serbs wanted: namely to have Kosovo all to themselves. So as soon as Madeleine's hired gunmen shifted the balance of power in Kosovo and forced the Serb army out, the Albanians, instead of hugging their Serb neighbors began killing and terrorizing them. The Serbs fled for their lives, and now the Albanians run Kosovo, except for a few all-Serb
Friday, July 19, 2019
The Publishing of Written Materials: Newspapers, Magazines and Books :: Publishing Publish Essays
The Publishing of Written Materials: Newspapers, Magazines and Books Publishing as an industry is in constant dialogue - a true back and forth - with the surrounding culture commercially, technologically, and artistically. It has changed greatly since the early 1800's and particularly during the 1900's. These changes have occurred in relationship to evolving aspects of American life, and so the factors one must consider are many: literacy and compulsory education, legal ownership of the actual words to be published, modes of production and distribution, availability of resources such as paper and printing equipment, the ideas which generate the written word, the presence of a reading public and the questions it asks, and surrounding social events - war, money, immigration, reconstruction of the South. A brief early history: Publishing begins in US when Stephen Day issues the Bay Psalm Book in 1640 from his press in Cambridge MA Printing under state control in colonial times, and bookselling seldom an independent vocation before 1850. In the early years of the Colonies, Cambridge, Mass., had the sole privilege of printing, but the monopoly was broken in 1674, when Marmaduke Johnson, who had come over to print an Indian Bible (1663), moved his press to Boston. Gradually others followed - Philadelphia had a press in 1685; New York City, in 1693. It was difficult for the colonial printer, as for any small printer, to produce large works because of a shortage of type; but patronage by the government helped to give his products a dignified style. Almanacs, primers, and law books were the staples of book production; works of theology formed the leading category. Until 1769 American printers bought their presses from England, but thereafter they acquired their equipment and supplies, including ink and paper, do mestically. Books were sold in various waysââ¬âby subscription, by the printer himself, by hawkers, and through shopkeepers. Though Massachusetts passed a law against hawkers in 1713, it carefully excluded book peddlers, who had a valuable function in rural areas.
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